Article
A monoclonal antibody (OT145) specific for the T cell antigen receptor V beta 6.7a allele detects an epitope related to a proposed superantigen-binding site.
Journal of immunology (Baltimore, Md. : 1950) - 15 Nov 1991
Prashar Y, Li Y, Kubinec J S, Jones N, Posnett D N
Abstract excerpt
A mAb, OT145, recognizes a TCR allotype encoded by one of two alleles of the V beta 6.7 gene. The peptide products of the two V beta 6.7 alleles differ because of nonconservative amino acid substitutions at positions 38 and 72. V beta 6.7a encodes ser38 and gly72, whereas V beta 6.7b encodes arg38 and glu72. We show here that the binding of mAb OT145 ot the beta-chain of TCR is lost when residue 72 of V beta 6.7a...
Topics
- Alleles
- Amino Acid Sequence
- Antibodies, Monoclonal
- Antibody Specificity
- Antigens
- Base Sequence
- Binding Sites
- Cell Line
- DNA Mutational Analysis
- Epitopes
- Humans