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Development of a high throughput transformation system for insertional mutagenesis in Magnaporthe oryzae.

Fungal genetics and biology : FG & B - 1 Oct 2007

Betts Melania F, Tucker Sara L, Galadima Natalia, Meng Yan, Patel Gayatri, Li Lei, Donofrio Nicole, Floyd Anna, Nolin Shelly, Brown Doug, Mandel M Alejandra, Mitchell Thomas K, Xu Jin-Rong, Dean Ralph A, Farman Mark L, Orbach Marc J

Abstract excerpt

Towards the goal of disrupting all genes in the genome of Magnaporthe oryzae and identifying their function, a collection of >55,000 random insertion lines of M. oryzae strain 70-15 were generated. All strains were screened to identify genes involved in growth rate, conidiation, pigmentation, auxotrophy, and pathogenicity. Here, we provide a description of the high throughput transformation and analysis pipeline...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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PMID: 17600737
DOI: 10.1016/j.fgb.2007.05.001

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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