Constructing unmarked gene deletions in Legionella pneumophila.

Abstract excerpt

The ability to construct recombinant alleles efficiently in strains of interest, particularly unmarked deletions that reduce the potential for polar effects, is essential to studies of both pathogenesis and basic bacterial physiology. Here we describe a three-phase approach for generating unmarked deletions in Legionella pneumophila by constructing a mutant allele in E. coli using λ-Red recombination, so-called...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Alleles
• Share to Escherichia coli
• Share to Gene Deletion