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Article

High-quality mutant libraries of Xanthomonas oryzae pv. oryzae and X. campestris pv. campestris generated by an efficient transposon mutagenesis system.

FEMS microbiology letters - 12 Sept 2003

Sun Qihong, Wu Wei, Qian Wei, Hu Jun, Fang Rongxiang, He Chaozu

Abstract excerpt

A novel transposon mutagenesis system for the phytopathogenic bacteria Xanthomonas oryzae pv. oryzae (Xoo) and X. campestris pv. campestris (Xcc) was developed using a Tn5-based transposome. A highly efficient transformation up to 10(6) transformants per microg transposon DNA was obtained. Southern blot and thermal asymmetric interlaced polymerase chain reaction analyses of Tn5 insertion sites suggested a random...

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PMID: 13129620
DOI: 10.1016/S0378-1097(03)00583-4

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Source receipt: pubmed, retrieved 7 Sept 2026.

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