Article
Gene deletion and allelic replacement in the filamentous fungus Podospora anserina.
Current genetics - 1 Apr 2008
El-Khoury Riyad, Sellem Carole H, Coppin Evelyne, Boivin Antoine, Maas Marc F P M, Debuchy Robert, Sainsard-Chanet Annie
Abstract excerpt
Gene replacement via homologous recombination is a fundamental tool for the analysis of gene function. However, this event is rare in organisms like the filamentous fungus Podospora anserina. We show here that deletion of the PaKu70 gene is an efficient strategy for improving gene manipulation in this organism. By using the DeltaPaKu70 strain, it is now possible (1) to produce deletion mutants with an efficiency...
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