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[Expression of a thermostable a-amylase mutant into Escherichia coli and Pichia pastoris].

Wei sheng wu xue bao = Acta microbiologica Sinica - 1 Oct 2006

Du Bing-Bing, Hao Shuai, Li Yun-Min, Yue Li-Li, Jiao Qing-Hua

Abstract excerpt

Alpha-amylase are of considerable commercial value. It can be produced by a wide variety of microorganisma. The alpha-amylase gene (amyE) from Bacillus licheniformis, which is widely used for the industrial hydrolysis of starch, was mutated (amyEM), then amplified by PCR and inserted into pBV220 and pPIC9k to obtain the recombinant vector pBV220-amyEM and pPIC9k-amyEM. These recombinant vectors were transformed...

Topics

Escherichia coli
Hydrogen-Ion Concentration
Mutation
Pichia
Recombinant Proteins
Temperature
alpha-Amylases

Source

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 17172038

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SDS

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-17172038-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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