Article
A new maltose-inducible high-performance heterologous expression system in Bacillus subtilis.
Biotechnology letters - 1 Aug 2017
Yue Jie, Fu Gang, Zhang Dawei, Wen Jianping
Abstract excerpt
OBJECTIVES: To improve heterologous proteins production, we constructed a maltose-inducible expression system in Bacillus subtilis. RESULTS: An expression system based on the promoter for maltose utilization constructed in B. subtilis. Successively, to improve the performance of the P malA -derived system, mutagenesis was employed by gradually shortening the length of P malA promoter and altering the spacing...
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