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Article

Expression and mutation of soybean beta-amylase in Escherichia coli.

European journal of biochemistry - 15 Jun 1993

Totsuka A, Fukazawa C

Abstract excerpt

The cDNA clones corresponding to soybean beta-amylase mRNA were isolated and sequenced. The cDNA contained an open-reading frame composed of 496 amino acids. The comparison of the amino acid sequence deduced from the cDNA with the N-terminal peptide sequence from mature enzyme proved that beta-amylase had no leader sequence. Employing the cDNA, the beta-amylase was directly synthesized in Escherichia coli by the...

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Identifiers

PMID: 8319688
DOI: 10.1111/j.1432-1033.1993.tb17981.x

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