Comparison of starch hydrolysis activity and thermal stability of two Bacillus licheniformis alpha-amylases and insights into engineering alpha-amylase variants active under acidic conditions.

Abstract excerpt

Bacillus licheniformis alpha-amylase (BLA) is widely used in various procedures of starch degradation in the food industry, and a BLA species with improved activity at higher temperature and under acidic conditions is desirable. Two BLA species, designated as PA and MA, have been isolated from the wild-type B. licheniformis strain and a mutant strain, respectively. In this study, their starch-hydrolysis activity...

Topics

• Amino Acid Sequence
• Bacillus
• Calorimetry, Differential Scanning
• Enzyme Stability
• Hot Temperature
• Hydrogen-Ion Concentration
• Hydrolysis
• Molecular Sequence Data
• Mutation
• Protein Engineering
• Starch
• Thermodynamics
• alpha-Amylases