Improvement of the acid stability of Bacillus licheniformis alpha amylase by error-prone PCR.

doi:10.1111/j.1365-2672.2012.05359.x

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Article

Improvement of the acid stability of Bacillus licheniformis alpha amylase by error-prone PCR.

Journal of applied microbiology - 1 Sept 2012

Liu Y H, Hu B, Xu Y J, Bo J X, Fan S, Wang J L, Lu F P

Abstract excerpt

AIMS: The purpose of this research was to obtain the mutant of Bacillus licheniformis alpha amylase (BLA) with an improved acid stability and elucidate the difference in catalytic mechanism under acidic conditions between wild-type and mutant BLAs. METHODS AND RESULTS: The stability of BLA under acid condition was enhanced through direct evolution using error-prone polymerase chain reaction. Two mutation sites,...

Topics

Acids
Bacillus
Enzyme Stability
Hot Temperature
Hydrogen-Ion Concentration
Mutagenesis, Site-Directed
Mutation
Polymerase Chain Reaction
Protein Engineering
Protein Structure, Secondary
alpha-Amylases

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PMID: 22681571
DOI: 10.1111/j.1365-2672.2012.05359.x

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