Article
Synergistic stimulation of EpsE ATP hydrolysis by EpsL and acidic phospholipids.
The EMBO journal - 10 Jan 2007
Camberg Jodi L, Johnson Tanya L, Patrick Marcella, Abendroth Jan, Hol Wim G J, Sandkvist Maria
Abstract excerpt
EpsE is a cytoplasmic component of the type II secretion system in Vibrio cholerae. Through ATP hydrolysis and an interaction with the cytoplasmic membrane protein EpsL, EpsE supports secretion of cholera toxin across the outer membrane. In this study, we have determined the effect of the cytoplasmic domain of EpsL (cyto-EpsL) and purified phospholipids on the ATPase activity of EpsE. Acidic phospholipids,...
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