In vitro protein translocation into inverted membrane vesicles prepared from Vibrio alginolyticus is stimulated by the electrochemical potential of Na+ in the presence of Escherichia coli SecA.

doi:10.1016/0014-5793(90)80751-4

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Article

In vitro protein translocation into inverted membrane vesicles prepared from Vibrio alginolyticus is stimulated by the electrochemical potential of Na+ in the presence of Escherichia coli SecA.

FEBS letters - 7 May 1990

Tokuda H, Kim Y J, Mizushima S

Abstract excerpt

A protein translocation system was reconstituted from inverted membrane vesicles prepared from Na+ pump-possessing Vibrio alginolyticus and purified Escherichia coli SecA. The translocation required ATP and was stimulated by the functioning of the Na+ pump, suggesting that the electrochemical pot...

Topics

Bacterial Outer Membrane Proteins
Bacterial Proteins
Biological Transport, Active
Cell Membrane
Escherichia coli
Kinetics
Mutation
Sodium
Vibrio

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