Article
Overproduction, solubilization, and reconstitution of the maltose transport system from Escherichia coli.
The Journal of biological chemistry - 15 Mar 1990
Davidson A L, Nikaido H
Abstract excerpt
Maltose is transported across the cytoplasmic membrane of Escherichia coli by a binding protein-dependent transport system. We observed a 10-fold increase in the level of transport activity in assays with membrane vesicles when the three membrane-associated components of the transport system (the...
Topics
- ATP-Binding Cassette Transporters
- Adenosine Triphosphate
- Bacterial Proteins
- Biological Transport, Active
- Carrier Proteins
- Cell Membrane
- Cloning, Molecular
- DNA Restriction Enzymes
- Escherichia coli
- Escherichia coli Proteins
- Gene Expression
- Liposomes
- Maltose
- Maltose-Binding Proteins
- Molecular Weight
- Monosaccharide Transport Proteins
- Mutation
- Plasmids