Article
Energy transduction between membranes. TonB, a cytoplasmic membrane protein, can be chemically cross-linked in vivo to the outer membrane receptor FepA.
The Journal of biological chemistry - 5 Aug 1993
Skare J T, Ahmer B M, Seachord C L, Darveau R P, Postle K
Abstract excerpt
TonB, a cytoplasmic membrane protein, couples cytoplasmic membrane protonmotive force to active transport across the outer membrane of Escherichia coli. In vivo cross-linking studies were initiated to analyze TonB interactions with other cell envelope proteins. Four TonB-specific cross-linked complexes were detected with apparent molecular masses of 195, 77, 59, and 43.5 kDa. The 195-kDa complex was shown to...
Topics
- Animals
- Bacterial Outer Membrane Proteins
- Bacterial Proteins
- Carrier Proteins
- Cross-Linking Reagents
- Energy Transfer
- Escherichia coli Proteins
- Female
- Formaldehyde
- Immunoblotting
- Membrane Proteins
- Mice
- Mice, Inbred BALB C
- Mutation