Article
Synthesis and properties of novel base-discriminating fluorescent (BDF) nucleosides.
Nucleic acids symposium series (2004) - 1 Jan 2005
Saito Yoshio, Hanawa Kazuo, Hayashi Keigo, Motegi Kaori, Okaoto Akimitsu, Saito Isao
Abstract excerpt
We designed a new type of pyrene-labeled base-discrimination fluorescent (BDF) nucleosides (Py)U, (Py)C, (8Py)A and (MePy)dA, which emitted strong fluorescence only when the bases opposite the BDF base are A, G, T and C, respectively. The DNA probes containing four different BDF bases enable us t...
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