Article
Clear distinction of purine bases on the complementary strand by a fluorescence change of a novel fluorescent nucleoside.
Journal of the American Chemical Society - 30 Apr 2003
Okamoto Akimitsu, Tainaka Kazuki, Saito Isao
Abstract excerpt
A new fluorescent nucleoside, benzopyridopyrimidine (BPP), which can sharply distinguish between A and G bases opposite BPP has been devised. The base-pairing degeneracy of BPP strongly contributes to the sharp fluorescence change that is dependent on the type of purine bases opposite BPP. The hybridization of an ODN probe containing BPP with a target DNA facilitates the judgment with the naked eye of the type of...
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