Site-directed mutagenesis of signal-recognition particle RNA. Identification of the nucleotides in helix 8 required for interaction with protein SRP19.

Abstract excerpt

The RNA component of signal recognition particle (SRP) consists of eight helices which form a functional unit with the proteins of the SRP. The primary binding site of the 19-kDa protein of SRP (SRP19) is a tetranucleotide loop (tetraloop) in helix 6 of the SRP RNA, but additional determinants are located in helix 8, which might play important roles in the assembly and the function of the particle. To determine...

Topics

• Base Composition
• Base Sequence
• Binding Sites
• Humans
• Molecular Sequence Data
• Molecular Weight
• Mutagenesis, Site-Directed
• Mutation
• Nucleic Acid Conformation
• Plasmids
• RNA