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Article

Structure-guided SCHEMA recombination of distantly related beta-lactamases.

Protein engineering, design & selection : PEDS - 1 Dec 2006

Meyer Michelle M, Hochrein Lisa, Arnold Frances H

Abstract excerpt

We constructed a library of beta-lactamases by recombining three naturally occurring homologs (TEM-1, PSE-4, SED-1) that share 34-42% sequence identity. Most chimeras created by recombining such distantly related proteins are unfolded due to unfavorable side-chain interactions that destabilize the folded structure. To enhance the fraction of properly folded chimeras, we designed the library using SCHEMA, a...

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Source

Identifiers

PMID: 17090554
DOI: 10.1093/protein/gzl045

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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