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Article

SCHEMA recombination of a fungal cellulase uncovers a single mutation that contributes markedly to stability.

The Journal of biological chemistry - 25 Sept 2009

Heinzelman Pete, Snow Christopher D, Smith Matthew A, Yu Xinlin, Kannan Arvind, Boulware Kevin, Villalobos Alan, Govindarajan Sridhar, Minshull Jeremy, Arnold Frances H

Abstract excerpt

A quantitative linear model accurately (R(2) = 0.88) describes the thermostabilities of 54 characterized members of a family of fungal cellobiohydrolase class II (CBH II) cellulase chimeras made by SCHEMA recombination of three fungal enzymes, demonstrating that the contributions of SCHEMA sequen...

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Identifiers

PMID: 19625252
DOI: 10.1074/jbc.C109.034058
PMCID: PMC2785310

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026. Prepared record publication-19625252-2026-09-02.2. Manifest publication-graph-cohort-2026-09-02.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 2 Sept 2026.

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