Article
Comparative genome sequencing of Escherichia coli allows observation of bacterial evolution on a laboratory timescale.
Nature genetics - 1 Dec 2006
Herring Christopher D, Raghunathan Anu, Honisch Christiane, Patel Trina, Applebee M Kenyon, Joyce Andrew R, Albert Thomas J, Blattner Frederick R, van den Boom Dirk, Cantor Charles R, Palsson Bernhard Ø
Abstract excerpt
We applied whole-genome resequencing of Escherichia coli to monitor the acquisition and fixation of mutations that conveyed a selective growth advantage during adaptation to a glycerol-based growth medium. We identified 13 different de novo mutations in five different E. coli strains and monitored their fixation over a 44-d period of adaptation. We obtained proof that the observed spontaneous mutations were...
Topics
- Adaptation, Physiological
- Culture Media
- Directed Molecular Evolution
- Escherichia coli
- Genome, Bacterial
- Genotype
- Glycerol
- Mutagenesis, Site-Directed
- Mutation
- Selection, Genetic
- Time Factors