Article
Evolution in Long-Term Stationary-Phase Batch Culture: Emergence of Divergent Escherichia coli Lineages over 1,200 Days.
mBio - 26 Jan 2021
Ratib Nicole R, Seidl Fabian, Ehrenreich Ian M, Finkel Steven E
Abstract excerpt
In natural environments, bacteria survive conditions of starvation and stress. Long-term batch cultures are an excellent laboratory system to study adaptation during nutrient stress because cells can incubate for months to years without the addition of nutrients. During long-term batch culture, cells adapt to acquire energy from cellular detritus, creating a complex and dynamic environment for mutants of...
Topics
- Adaptation, Physiological
- Alleles
- Batch Cell Culture Techniques
- Culture Media
- Escherichia coli
- Escherichia coli Proteins
- Evolution, Molecular
- Mutation
- Phenotype
- Time Factors
- Whole Genome Sequencing