Article
High-throughput laboratory evolution reveals evolutionary constraints in Escherichia coli.
Nature communications - 24 Nov 2020
Maeda Tomoya, Iwasawa Junichiro, Kotani Hazuki, Sakata Natsue, Kawada Masako, Horinouchi Takaaki, Sakai Aki, Tanabe Kumi, Furusawa Chikara
Abstract excerpt
Understanding the constraints that shape the evolution of antibiotic resistance is critical for predicting and controlling drug resistance. Despite its importance, however, a systematic investigation of evolutionary constraints is lacking. Here, we perform a high-throughput laboratory evolution of Escherichia coli under the addition of 95 antibacterial chemicals and quantified the transcriptome, resistance, and...
Topics
- Anti-Bacterial Agents
- Drug Resistance, Multiple, Bacterial
- Escherichia coli
- Evolution, Molecular
- Genes, Bacterial
- Genotype
- Microbial Sensitivity Tests
- beta-Lactam Resistance