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Article

Altered -3 substrate specificity of Escherichia coli signal peptidase 1 mutants as revealed by screening a combinatorial peptide library.

The Journal of biological chemistry - 5 Jan 2007

Ekici Ozlem Dogan, Karla Andrew, Paetzel Mark, Lively Mark O, Pei Dehua, Dalbey Ross E

Abstract excerpt

Signal peptidase functions to cleave signal peptides from preproteins at the cell membrane. It has a substrate specificity for small uncharged residues at -1 (P1) and aliphatic residues at the -3 (P3) position. Previously, we have reported that certain alterations of the Ile-144 and Ile-86 residues in Escherichia coli signal peptidase I (SPase) can change the specificity such that signal peptidase is able to...

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PMID: 17077081
DOI: 10.1074/jbc.M608779200

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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