Article
Use of site-directed mutagenesis to define the limits of sequence variation tolerated for processing of the M13 procoat protein by the Escherichia coli leader peptidase.
Biochemistry - 24 Dec 1991
Shen L M, Lee J I, Cheng S Y, Jutte H, Kuhn A, Dalbey R E
Abstract excerpt
Leader peptidase cleaves the leader sequence from the amino terminus of newly made membrane and secreted proteins after they have translocated across the membrane. Analysis of a large number of leader sequences has shown that there is a characteristic pattern of small apolar residues at -1 and -3 (with respect to the cleavage site) and a helix-breaking residue adjacent to the central apolar core in the region -4...
Topics
- Amino Acid Sequence
- Base Sequence
- Capsid
- Coliphages
- Endopeptidases
- Escherichia coli
- Genetic Variation
- Membrane Proteins
- Molecular Sequence Data
- Mutagenesis, Site-Directed