Article
The role of the conserved box E residues in the active site of the Escherichia coli type I signal peptidase.
The Journal of biological chemistry - 3 Mar 2000
Klenotic P A, Carlos J L, Samuelson J C, Schuenemann T A, Tschantz W R, Paetzel M, Strynadka N C, Dalbey R E
Abstract excerpt
Type I signal peptidases are integral membrane proteins that function to remove signal peptides from secreted and membrane proteins. These enzymes carry out catalysis using a serine/lysine dyad instead of the prototypical serine/histidine/aspartic acid triad found in most serine proteases. Site-directed scanning mutagenesis was used to obtain a qualitative assessment of which residues in the fifth conserved...
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