Article
Rational design of p53, an intrinsically unstructured protein, for the fabrication of novel molecular sensors.
The Journal of biological chemistry - 21 Oct 2005
Geddie Melissa L, O'Loughlin Taryn L, Woods Kristen K, Matsumura Ichiro
Abstract excerpt
The dominant paradigm of protein engineering is structure-based site-directed mutagenesis. This rational approach is generally more effective for the engineering of local properties, such as substrate specificity, than global ones such as allostery. Previous workers have modified normally unregulated reporter enzymes, including beta-galactosidase, alkaline phosphatase, and beta-lactamase, so that the engineered...
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