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Article

Asymmetric PCR-based strategy for genetic analysis of the p53 tumor suppressor gene in cell lines and tumor tissues.

Neoplasma - 1 Jan 1991

Bártek J, Iggo R, Vojtĕsek B, Lane D P

Abstract excerpt

A novel strategy for genetic analysis of the p53 tumor suppressor gene is described, based on direct sequencing of the asymmetric polymerase chain reaction (PCR) products. A set of 10 PCR primers was designed which allows to amplify and sequence highly conserved regions of the molecule, i.e. the target areas of p53 mutations. The stepwise optimization of RNA isolation, cDNA synthesis, PCR amplifications and...

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PMID: 1707144

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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