Asymmetric PCR-based strategy for genetic analysis of the p53 tumor suppressor gene in cell lines and tumor tissues.

Article

Asymmetric PCR-based strategy for genetic analysis of the p53 tumor suppressor gene in cell lines and tumor tissues.

Neoplasma - 1 Jan 1991

Bártek J, Iggo R, Vojtĕsek B, Lane D P

Abstract excerpt

A novel strategy for genetic analysis of the p53 tumor suppressor gene is described, based on direct sequencing of the asymmetric polymerase chain reaction (PCR) products. A set of 10 PCR primers was designed which allows to amplify and sequence highly conserved regions of the molecule, i.e. the...

Topics

Base Sequence
DNA
Genes, Tumor Suppressor
Genes, p53
Humans
Molecular Sequence Data
Mutation
Neoplasms
Oligonucleotides
Polymerase Chain Reaction
RNA
Tumor Cells, Cultured

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.