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Article

Construction of saccharide-modified DNAs by DNA polymerase.

Bioorganic & medicinal chemistry letters - 15 Jan 2007

Matsui Masayuki, Nishiyama Yoshitaka, Ueji Shin-ichi, Ebara Yasuhito

Abstract excerpt

Novel deoxyribonucleotide triphosphates bearing maltose or lactose groups were synthesized as substrates for DNA polymerase. The incorporation efficiencies of these modified substrates were investigated in both primer extension reactions and PCR. The stability and conformation of saccharide-modified dsDNAs were assessed by UV absorbance melting experiments and CD analysis. Enzymatic incorporation of...

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PMID: 17064895
DOI: 10.1016/j.bmcl.2006.10.020

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