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Methods for Metabolic Engineering of Thermoanaerobacterium saccharolyticum.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2020

Hon Shuen, Tian Liang, Zheng Tianyong, Cui Jingxuan, Lynd Lee R, Olson Daniel G

Abstract excerpt

In this work, we describe genetic tools and techniques for engineering Thermoanaerobacterium saccharolyticum. In particular, the T. saccharolyticum transformation protocol and the methods for selecting for transformants are described. Methods for determining strain phenotypes are also presented.

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PMID: 32720144
DOI: 10.1007/978-1-0716-0195-2_3

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