Article
Expression and purification of a single-chain Type IV restriction enzyme Eco94GmrSD and determination of its substrate preference.
Scientific reports - 19 May 2015
He Xinyi, Hull Victoria, Thomas Julie A, Fu Xiaoqing, Gidwani Sonal, Gupta Yogesh K, Black Lindsay W, Xu Shuang-yong
Abstract excerpt
The first reported Type IV restriction endonuclease (REase) GmrSD consists of GmrS and GmrD subunits. In most bacteria, however, the gmrS and gmrD genes are fused together to encode a single-chain protein. The fused coding sequence for ECSTEC94C_1402 from E. coli strain STEC_94C was expressed in T7 Express. The protein designated as Eco94GmrSD displays modification-dependent ATP-stimulated REase activity on T4...
Topics
- Catalytic Domain
- DNA Restriction Enzymes
- Enzyme Activation
- Gene Expression
- Ions
- Metals
- Mutation
- Protein Interaction Domains and Motifs
- Protein Subunits
- Recombinant Fusion Proteins