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Article

Optimizing protein V untranslated region sequence in M13 phage for increased production of single-stranded DNA for origami.

Nucleic acids research - 21 Jun 2021

Lee Bo-Young, Lee Jaewon, Ahn Dong June, Lee Seungwoo, Oh Min-Kyu

Abstract excerpt

DNA origami requires long scaffold DNA to be aligned with the guidance of short staple DNA strands. Scaffold DNA is produced in Escherichia coli as a form of the M13 bacteriophage by rolling circle amplification (RCA). This study shows that RCA can be reconfigured by reducing phage protein V (pV) expression, improving the production throughput of scaffold DNA by at least 5.66-fold. The change in pV expression was...

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Identifiers

PMID: 34110422
DOI: 10.1093/nar/gkab455
PMCID: PMC8216270

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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