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Article

Improvement of cellulase activity using error-prone rolling circle amplification and site-directed mutagenesis.

Journal of microbiology and biotechnology - 1 May 2012

Vu Van Hanh, Kim Keun

Abstract excerpt

Improvement of endoglucanase activity was accomplished by utilizing error-prone rolling circle amplification, supplemented with 1.7 mM MnCl2. This procedure generated random mutations in the Bacillus amyloliquefaciens endoglucanase gene with a frequency of 10 mutations per kilobase. Six mutated endoglucanase genes, recovered from six colonies, possessed endoglucanase activity between 2.50- and 3.12-folds higher...

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Identifiers

PMID: 22561853
DOI: 10.4014/jmb.1107.07033

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