Article
Interrogation of multimeric DNA amplification products by competitive primer extension using bst DNA polymerase (large fragment).
BioTechniques - 1 Nov 2001
Voisey J, Hafner G J, Morris C P, van Daal A, Giffard P M
Abstract excerpt
Linear dsDNA composed of tandem repeats may be exponentially amplified by the strongly strand-displacing Bst DNA polymerase (large fragment) and two primers specific for opposite strands. When the repetitive DNA is derivedfrom rolling circle replication of a circular template, the reaction is termed cascade rolling circle amplification (CRCA). We have developed a variant of CRCA in which one primer is attached to...
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