Article
Partial transformation of human thyroid epithelial cells by mutant Ha-ras oncogene.
Oncogene - 1 Dec 1990
Lemoine N R, Staddon S, Bond J, Wyllie F S, Shaw J J, Wynford-Thomas D
Abstract excerpt
We have previously shown that activation of ras oncogenes by mutation is a frequent early event in human thyroid neoplasia. Using amphotropic retroviral vectors to achieve gene transfer, we demonstrate here that human primary thyroid epithelial cells can be partially transformed by an activated cellular or viral Ha-ras oncogene, in the absence of a cooperating oncogene. The transformation event induced by ras...
Topics
- Cell Transformation, Neoplastic
- Epithelial Cells
- Epithelium
- Genes, ras
- Genetic Vectors
- Humans
- Immunoblotting
- Immunohistochemistry
- Keratins
- Mutation
- Thyroglobulin
- Thyroid Gland