Article
A phenotypically and karyotypically stable human thyroid epithelial line conditionally immortalized by SV40 large T antigen.
Cancer research - 15 May 1992
Wyllie F S, Bond J A, Dawson T, White D, Davies R, Wynford-Thomas D
Abstract excerpt
Primary cultures of normal human neonatal thyroid follicular cells were transfected with a plasmid expressing a temperature-sensitive (tsA58) mutant of SV40 large T antigen. An epithelial cell line, designated B-thy-ts.1, was obtained which showed tight temperature-dependent growth. In sharp contrast to previous such lines, which were derived from adult thyroid, B-thy-ts.1 has retained a well-differentiated...
Topics
- Antigens, Polyomavirus Transforming
- Cell Adhesion
- Cell Differentiation
- Cell Division
- Cell Transformation, Viral
- Cells, Cultured
- Epithelial Cells
- Genetic Variation
- Growth Substances
- Humans
- Immunohistochemistry