Article
Metabolic characterisation of E. coli citrate synthase and phosphoenolpyruvate carboxylase mutants in aerobic cultures.
Biotechnology letters - 1 Dec 2006
De Maeseneire S L, De Mey M, Vandedrinck S, Vandamme E J
Abstract excerpt
E. coli is still one of the most commonly used hosts for protein production. However, when it is grown with excess glucose, acetate accumulation occurs. Elevated acetate concentrations have an inhibitory effect on growth rate and recombinant protein yield, and thus elimination of acetate formation is an important aim towards industrial production of recombinant proteins. Here we examine if over-expression of...
Topics
- Acetates
- Biotechnology
- Citrate (si)-Synthase
- Cloning, Molecular
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Genetic Techniques
- Glucose
- Hydrogen-Ion Concentration
- Metabolism
- Microbiological Techniques
- Models, Biological
- Mutation
- Phosphoenolpyruvate Carboxylase