Article
Strategies in high-level expression of recombinant protein in Escherichia coli.
Annals of the New York Academy of Sciences - 2 May 1994
San K Y, Bennett G N, Aristidou A A, Chou C H
Abstract excerpt
The accumulation of acetate is one of the most commonly encountered problems in attaining high levels of recombinant protein production using E. coli. Two different approaches are examined to reduce the rate of acetate formation. The effects of reduced acetate accumulation on recombinant protein...
Topics
- Acetates
- Acetic Acid
- Acetolactate Synthase
- Bacillus subtilis
- Biotechnology
- Escherichia coli
- Fluoroacetates
- Gene Expression
- Genetic Engineering
- Glucose
- Mutation
- Plasmids
- Recombinant Proteins
- beta-Galactosidase