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Article

Eliminating acetate formation improves citramalate production by metabolically engineered Escherichia coli.

Microbial cell factories - 21 Jun 2017

Parimi Naga Sirisha, Durie Ian A, Wu Xianghao, Niyas Afaq M M, Eiteman Mark A

Abstract excerpt

BACKGROUND: Citramalate, a chemical precursor to the industrially important methacrylic acid (MAA), can be synthesized using Escherichia coli overexpressing citramalate synthase (cimA gene). Deletion of gltA encoding citrate synthase and leuC encoding 3-isopropylmalate dehydratase were critical to achieving high citramalate yields. Acetate is an undesirable by-product potentially formed from pyruvate and...

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Identifiers

PMID: 28637476
DOI: 10.1186/s12934-017-0729-2
PMCID: PMC5480221

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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