Induced reversion of a spontaneous point mutation within the Chinese hamster HPRT gene to the wild-type sequence.

doi:10.1093/mutage/5.6.605

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Article

Induced reversion of a spontaneous point mutation within the Chinese hamster HPRT gene to the wild-type sequence.

Mutagenesis - 1 Nov 1990

Rossiter B J, Muzny D M, Hampson I, Caskey C T, Fox M

Abstract excerpt

The Chinese hamster hypoxanthine-guanine phosphoribosyltransferase (HPRT)-deficient cell line TG15 produces apparently normal HPRT mRNA by northern analysis and was therefore presumed to contain a point mutation within the coding region. Sequencing cDNA from the TG15 cell line revealed an A to G...

Topics

Animals
Base Sequence
Cell Line
Cricetinae
Cricetulus
DNA
Genes
Hypoxanthine Phosphoribosyltransferase
Molecular Sequence Data
Mutation
Oligonucleotide Probes
Poly A
RNA
RNA, Messenger
Restriction Mapping

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