Article
Deoxyribonucleic acid preparation in polymerase chain reaction genotyping of transgenic mice.
Comparative medicine - 1 Jun 2000
Zimmermann K, Schwarz H P, Turecek P L
Abstract excerpt
BACKGROUND AND PURPOSE: In an attempt to find a rapid and reproducible method for routine polymerase chain reaction genotyping of transgenic mice, two novel approaches were developed. METHODS: One approach allowed reproducible amplification from crude lysates of tail snips, using a thermally activated polymerase. In a second approach, for situations in which non-invasive techniques are necessary, oral swab...
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