Article
Evaluation of a reliable and cost-effective method of DNA isolation for mouse genotyping.
Biotechnology letters - 1 Apr 2013
Sysol Justin R, Kempf Carrie, Helton Michelle N, Dong Yangbasai, Zhu Daocheng, Sun Haiying, Garcia Joe G N, Machado Roberto F, Chen Jiwang
Abstract excerpt
Genotyping is commonly used to define specific gene alterations or the presence of transgenes in mice. This procedure is typically done using DNA isolated from mouse tail tissue. Although there are commercially available kits for tail DNA isolation, they can be time consuming and costly for routine genotyping. In this study, we describe a rapid, "crude" DNA isolation method using mouse tail tissue and compare it...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.