Article
Non-invasive genotyping of transgenic animals using fecal DNA.
Lab animal - 20 Mar 2012
Chen Zhidong, Mantha Rebecca R, Chen Janice S, Slivano Orazio J, Takahashi Hideko
Abstract excerpt
For genotyping of transgenic animals, many IACUC guidelines recommend the use of fecal DNA when possible because this approach is non-invasive. Existing methods for extracting fecal DNA may be costly or involve the use of toxic organic solvents. Furthermore, feces contain an abundance of PCR inhibitors that may hinder DNA amplification when they are co-purified with fecal DNA. Here the authors describe a...
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