Article
Immunochemical characterization of six monoclonal antibodies to human apolipoprotein A-I: epitope mapping and expression.
Journal of lipid research - 1 Mar 1990
Marcovina S, Fantappie S, Zoppo A, Franceschini G, Catapano A L
Abstract excerpt
We have produced and characterized six murine monoclonal antibodies to human apolipoprotein A-I named A-I-9, A-I-12, A-I-15, A-I-16, A-I-19, and A-I-57. All monoclonal antibodies were specific for apolipoprotein A-I and bound between 55% and 100% of 125I-labeled high density lipoproteins (HDL) in a fluid phase radioimmunoassay. All antibodies possessed a higher affinity to apoA-I in HDL than to free, delipidated...
Topics
- Animals
- Antibodies, Monoclonal
- Antibody Specificity
- Apolipoprotein A-I
- Apolipoproteins A
- Binding, Competitive
- Electrophoresis, Polyacrylamide Gel
- Epitopes
- Humans
- Immunoblotting
- Lipoproteins, HDL