Article
Immunological discrimination between the human apolipoprotein E2(Arg158----Cys) and E3 isoforms.
Journal of lipid research - 1 Feb 1992
Gerritse K, de Knijff P, van Ierssel G, Havekes L M, Frants R R, Schellekens M M, Zegers N D, Claassen E, Boersma W J
Abstract excerpt
A specific anti-apoE2(Arg158----Cys) monoclonal antibody was raised by means of immunization of mice with a variant specific synthetic peptide. The peptide sequences used were homologous to apolipoprotein E of human and mouse. Consequently, the mouse immune system was tolerant to most of the sele...
Topics
- Amino Acid Sequence
- Animals
- Antibodies, Monoclonal
- Antibody Specificity
- Apolipoprotein E2
- Apolipoprotein E3
- Apolipoproteins E
- Arginine
- Cysteine
- Enzyme-Linked Immunosorbent Assay
- Epitopes
- Female
- Humans
- Immunoblotting
- Isoelectric Focusing
- Mice
- Mice, Inbred BALB C
- Molecular Sequence Data