Localization of residues that confer antibody binding specificity using human chorionic gonadotropin/luteinizing hormone beta subunit chimeras and mutants.

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Article

Localization of residues that confer antibody binding specificity using human chorionic gonadotropin/luteinizing hormone beta subunit chimeras and mutants.

The Journal of biological chemistry - 25 May 1990

Moyle W R, Matzuk M M, Campbell R K, Cogliani E, Dean-Emig D M, Krichevsky A, Barnett R W, Boime I

Abstract excerpt

The glycoprotein hormones are a family of conserved heterodimeric proteins which share a common alpha subunit but differ in their hormone-specific beta subunits. We used chimeras of human chorionic gonadotropin (hCG) and luteinizing hormone (hLH) beta subunits to identify residues which enable monoclonal antibodies (mAb) to distinguish the two hormones. The LH beta-CG beta chimeras appeared to fold similar to hCG...

Topics

Amino Acid Sequence
Antibodies, Monoclonal
Antibody Specificity
Blotting, Western
Chimera
Chorionic Gonadotropin
Chorionic Gonadotropin, beta Subunit, Human
Humans
Immunoassay
Luteinizing Hormone

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Models, Molecular

Models, Structural

Molecular Sequence Data

Mutation

Peptide Fragments

Protein Conformation

Sequence Homology, Nucleic Acid

Source

Open in PubMed

Identifiers

PMID: 1692832

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1692832-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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