Article
The human chorionic gonadotropin-beta arginine68 to glutamic acid substitution fixes the conformation of the C-terminal peptide.
Molecular endocrinology (Baltimore, Md.) - 1 Jul 2005
Charrel-Dennis Marie, Terrazzini Nadia, McBride Jeffrey D, Kaye Paul, Martensen Pia M, Justesen Just, Berger Peter, Lapthorn Adrian, Kelly Charles, Roitt Ivan M, Delves Peter J, Lund Torben
Abstract excerpt
Wild-type human chorionic gonadotropin (hCG) has been used as a contraceptive vaccine. However, extensive sequence homology with LH elicits production of cross-reactive antibodies. Substitution of arginine(68) of the beta-subunit (hCG(beta)) with glutamic acid (R68E) profoundly reduces the cross-reactivity while refocusing the immune response to the hCG(beta)-specific C-terminal peptide (CTP). To investigate the...
Topics
- Amino Acid Substitution
- Animals
- Antibodies
- Antibodies, Monoclonal
- Arginine
- Chorionic Gonadotropin, beta Subunit, Human
- Cross Reactions
- Female
- Glutamic Acid
- Humans
- Immunization
- Luteinizing Hormone