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Article

Thermodynamics of single peptide bond cleavage in bovine pancreatic trypsin inhibitor (BPTI).

Protein science : a publication of the Protein Society - 1 Apr 2002

Buczek Olga, Krowarsch Daniel, Otlewski Jacek

Abstract excerpt

A major goal of this paper was to estimate a dynamic range of equilibrium constant for the opening of a single peptide bond in a model protein, bovine pancreatic trypsin inhibitor (BPTI). Ten mutants of BPTI containing a single Xaa-->Met substitution introduced in different parts of the molecule were expressed in Escherichia coli. The mutants were folded, purified to homogeneity, and cleaved with cyanogen bromide...

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Identifiers

PMID: 11910035
DOI: 10.1110/ps.4460102
PMCID: PMC2373530

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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