Article
Function of normal and mutated gamma-globin gene promoters in electroporated K562 erythroleukemia cells.
Blood - 15 Feb 1990
Ulrich M J, Ley T J
Abstract excerpt
We examined the importance of cis-acting regulatory elements of the human gamma-globin gene promoter in a cell line (K562) where this gene normally functions. A gamma-Globin promoter fragments were fused to the neomycin phosphotransferase (neoR) gene in a plasmid-based vector and transiently transfected by electroporation into K562 cells. Correctly initiated "A gamma-neo" transcripts were detected with an S1...
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