Article
Effect of different promoters on expression of genes introduced into hematopoietic and marrow stromal cells by electroporation.
Experimental hematology - 1 Feb 1990
Keating A, Horsfall W, Hawley R G, Toneguzzo F
Abstract excerpt
Electroporation is a simple and relatively efficient means of introducing genes into hematopoietic cells. However, achieving and maintaining high levels of gene expression in transfected hematopoietic progenitor cells remains problematic. In order to address this problem we examined the effect of different viral and cellular promoters on the transient expression of reporter genes transferred into K562, KG1a, and...
Topics
- Bone Marrow
- Bone Marrow Cells
- Cell Line
- Chloramphenicol O-Acetyltransferase
- Gene Expression Regulation, Enzymologic
- Gene Expression Regulation, Leukemic
- Genetic Techniques
- Genetic Vectors
- Hematopoietic System
- Humans