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Article

The trp1- delta FA designer deletion for PCR-based gene functional analysis in Saccharomyces cerevisiae.

Yeast (Chichester, England) - 1 Dec 1999

Horecka J, Jigami Y

Abstract excerpt

PCR-based gene deletion and modification are now common techniques for rapid gene manipulation in the yeast Saccharomyces cerevisiae. The techniques work best when the host strain lacks sequence homology to the PCR-amplified selectable markers. One of the most versatile sets of PCR deletion/modif...

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Source

Identifiers

PMID: 10590465
DOI: 10.1002/(SICI)1097-0061(199912)15:16<1769::AID-YEA495>3.0.CO;2-M

Genes

GAL

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026. Prepared record publication-10590465-2026-09-04.2. Manifest publication-graph-cohort-2026-09-04.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 4 Sept 2026.

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